Recombinant SARS-Cov-2 Spike S1 protein (69/70 deletion, 144Y deletion, N501Y, A570D, D614G, P618H), His (HEK293)

• Catalog Number: orb1516696
• Category: Proteins
• Description: Recombinant SARS-Cov-2 Spike S1 protein (69/70 deletion, 144Y deletion, N501Y, A570D, D614G, P618H), His (HEK293)
• Tag: His
• Concentration: ＞0.5 mg/ml
• Form/Appearance: Lyophilized or Liquid
• Purity: > 95% as determined by SDS-PAGE
• MW: 110-130 kDa
• Target: Spike glycoprotein
• Protein Sequence: 14-685/1273
• Expression System: HEK293 cell
• Biological Activity: Yes
• Endotoxins: Not analyzed
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 10mM TBS (pH7.4).
• Alternative names: SARS-CoV-2 spike protein; 2019-nCOV Spike protein;
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: orb1516578) at 2 µg/ml (100 ul/Well) can bind SARS-CoV-2 Spike S1 (69/70 deletion, 144Y deletion, N501Y, A570D, D614G, P618H), His Tag (Cat: orb1516696), the EC50 is 131.51 ng/ml.

The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.