Recombinant SARS-Cov-2 Spike RBD protein (K417T, E484K, N501Y), His (HEK293)

• Catalog Number: orb1516698
• Category: Proteins
• Description: Recombinant SARS-Cov-2 Spike RBD protein (K417T, E484K, N501Y), His (HEK293)
• Target: Spike glycoprotein
• Tag: His
• Form/Appearance: Lyophilized or Liquid
• Concentration: ＞0.5 mg/ml
• Buffer/Preservatives: PBS (pH7.4).
• Purity: > 90% as determined by SDS-PAGE
• Protein Sequence: 319-541/1273
• MW: 36 kDa
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expression System: HEK293 cell
• Endotoxins: Not analyzed
• Biological Activity: Yes
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Alternative names: SARS-CoV-2 spike protein; 2019-nCOV Spike protein;
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: orb1516578) at 2 µg/ml (100 ul/Well) can bind SARS-CoV-2 Spike RBD (K417T, E484K, N501Y), His Tag (Cat: orb1516698), the EC50 is 26.96 ng/ml.

The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.