Recombinant SARS-Cov-2 Spike RBD protein, His & Avi (HEK293)

• Catalog Number: orb1516575
• Category: Proteins
• Description: Recombinant SARS-Cov-2 Spike RBD protein, His & Avi (HEK293)
• Tag: His & Avi
• Concentration: ＞0.5 mg/ml
• Form/Appearance: Lyophilized or Liquid
• Purity: > 95% as determined by SDS-PAGE
• MW: 34 kDa
• Target: Spike glycoprotein
• Protein Sequence: 319-532/1273
• Expression System: HEK293 cell
• Biological Activity: Yes
• Endotoxins: Not analyzed
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from 0.22um filtered solution in 2X PBS (pH7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Alternative names: coronavirus spike Protein, 2019-nCoV; cov spike Pr
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: orb1516578) at 2 µg/ml (100 ul/Well) can bind SARS-CoV-2 Spike RBD, His-Avi Tag (Cat: orb1516575), the EC50 is 103.11 ng/ml.

The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.