Recombinant SARS-Cov-2 (Omicron, B.1.1.529) Spike RBD protein (G339D, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H), C-His (HEK293)

• Catalog Number: orb1516623
• Category: Proteins
• Description: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) Spike RBD protein (G339D, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H), C-His (HEK293)
• Tag: C-His
• Concentration: ＞0.5 mg/ml
• Form/Appearance: Lyophilized or Liquid
• Purity: > 90% as determined by SDS-PAGE
• MW: 36 kDa
• Target: Spike glycoprotein
• Protein Sequence: 319-537/1273
• Expression System: HEK293 cell
• Biological Activity: Not tested
• Endotoxins: Not analyzed
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: PBS (pH7.4).
• Alternative names: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) Spike
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: orb1516578) at 2 µg/mL (100 µl/Well) can bind SARS-CoV-2 Spike RBD (G339D, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H), His Tag (Cat: orb1516623), the EC50 is 37 ng/ml.

The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.