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Description
Research Area
Musculoskeletal & Connective Tissue Research
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Rhesus macaque |
| Biological Activity | ELISA: Immobilized Rhesus Macaque TROP-2-His(Cat#orb2993743) at 1μg/ml (100 μl/well) can bind Anti-Human TROP-2 mAb(Cat#orb2993115).The ED50 of Anti-Human TROP-2 mAb(Cat#orb2993115) is 4.15 ng/ml.. BLI: Loaded Anti-Human TROP-2 mAb-Fc(Cat#orb2993115) on Protein A Biosensor, can bind Rhesus Macaque TROP-2-His(Cat#orb2993743) with an affinity constant of 0.29 nM as determined in BLI assay. Loaded Anti-Human TROP-2 mAb-Fc(Cat#orb2993115) on Protein A Biosensor, can bind Rhesus Macaque TROP-2-His(Cat#orb2993743) with an affinity constant of 0.29 nM as determined in BLI assay. |
| Tag | C-6His |
| Expression Region | His27-Arg272 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 28.7 KDa. Observed: 38-45 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor-associated calcium signal transducer 2; Membrane component chromosome 1 surface marker 1; Cell surface glycoprotein Trop-2; TACSTD2; TROP2
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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