Recombinant Rhesus Macaque Interleukin-3

SKU: orb1906221

Description

Recombinant Rhesus Macaque Interleukin-3

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Assay #1: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2 x105 IU/mg.Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107 IU/mg.
• Molecular Weight: Approximately 14.0 kDa, a single non-glycosylated polypeptide chain containing 124 amino acids.
• Protein Sequence: APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ
• Purity: > 98 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1 EU/ug of rRhIL-3 as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor

Quick Database Links

UniProt

P25140