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Recombinant Rat Vascular Endothelial Growth Factor 164
SKU: orb1906114
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Description
Research Area
Cardiovascular Research; Stem Cell & Developmental Biology
Images & Validation
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 �� 105 IU/mg. |
| Molecular Weight | Approximately 38.7 kDa, a disulfide-linked homodimeric protein, consisting of two 165 amino acid polypeptide chains with Met at N-terminus. |
| Protein Sequence | MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rRtVEGF164 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 um filtered concentrated solution in PBS. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−VPF
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−Recombinant Rat VEGF 164 Protein [orb2994452]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.2 KDa. Observed: 18-23 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Rat Vascular Endothelial Growth Factor 164, Yeast [orb1906113]
> 95 % by SDS-PAGE and 90% by SEC-HPLC analyses.
Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.
Yeast
10 μg, 100 μg, 500 μg
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Recombinant Rat Vascular Endothelial Growth Factor 164 (orb1906114)
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