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Catalog Number | orb1905974 |
---|---|
Category | Proteins |
Description | Recombinant Rat Prolactin |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 22.6 kDa, a single non-glycosylated polypeptide chain containing 197 amino acids. |
UniProt ID | P01237 |
Protein Sequence | LPVCSGGDCQ TPLPELFDRV VMLSHYIHTL YTDMFIEFDK QYVQDREFIA KAINDCPTSS LATPEDKEQA QKVPPEVLLN LILSLVHSWN DPLFQLITGL GGIHEAPDAI ISRAKEIEEQ NKRLLEGIEK IISQAYPEAK GNEIYLVWSQ LPSLQGVDEE SKDLAFYNNI RCLRRDSHKV DNYLKFLRCQ IVHKNNC |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x106 IU/mg. |
Endotoxins | Less than 0.1 EU/ugof rRtProlactin as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
12.2 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
15.7 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
26.6 kDa | |
E.coli |
SDS-PAGE | |
Unconjugated | |
> 98% by SDS-PAGE and HPLC analyses. | |
22.6 kDa | |
E. coli |
Greater than 90% as determined by SDS-PAGE. | |
13.7 kDa | |
E.coli |
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