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Catalog Number | orb1906107 |
---|---|
Category | Proteins |
Description | Recombinant Rat Mesencephalic Astrocyte-Derived Neurotrophic Factor |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids. |
UniProt ID | P0C5H9 |
Protein Sequence | LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg. |
Endotoxins | Less than 0.1 EU/ug of rRtMANF as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95% by SDS-PAGE and HPLC analyses. | |
18.2 kDa |
Unconjugated | |
95% | |
20.0 kDa | |
Human MANF Protein, His Tag (orb1570930) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Leu 182 (Accession # P55145). |
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