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Catalog Number | orb1952577 |
---|---|
Category | Proteins |
Description | Recombinant Rat Leukemia Inhibitory Factor |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4. |
Purity | > 96 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids. |
UniProt ID | P17777 |
Protein Sequence | SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF |
Expression System | Escherichia coli |
Biological Activity | Test in process. |
Endotoxins | Less than 0.1 EU/ug of rRtLIF as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
23.5 kDa | |
Mammalian cell |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96 % by SDS-PAGE and HPLC analyses | |
20.3 kDa |
≥95% | |
26.8 kDa | |
CHO Cell |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96 % by SDS-PAGE and HPLC analyses | |
24.3 kDa |