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Description
Research Area
Immunology & Inflammation
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−Item 1 of 1
Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg. |
| Molecular Weight | Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| Protein Sequence | MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rRtIL-1β as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP
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Recombinant Rat Interleukin-1 beta (orb1906141)
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