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Catalog Number | orb1906119 |
---|---|
Category | Proteins |
Description | Recombinant Rat Fibroblast Growth Factor 9 |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH 8.0. |
Purity | > 95 % by SDS-PAGE and HPLC analyses. |
Protein Sequence | LGEVGSYFGV QDAVPFGNVP VLPVDSPVLL SDHLGQSEAG GLPRGPAVTD LDHLKGILRR RQLYCRTGFH LEIFPNGTIQ GTRKDHSRFG ILEFISIAVG LVSIRGVDSG LYLGMNEKGE LYGSEKLTQE CVFREQFEEN WYNTYSSNLY KHVDTGRRYY VALNKDGTPR EGTRTKRHQK FTHFLPRPVD PDKVPELYKD ILSQS |
UniProt ID | P36364 |
MW | Approximately 23.1 kDa, a single non-glycosylated polypeptide chain containing 205 amino acids. |
Expression System | Escherichia coli |
Endotoxins | Less than 1 EU/ug of rRtFGF-9-1 as determined by LAL method. |
Source | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Alternative names | GAF, Glia-activating factor, HBGF-9 |
Note | For research use only |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95% by SDS-PAGE and HPLC analyses | |
23.3 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
22.4 kDa | |
E.Coli |
> 95% as analyzed by SDS-PAGE and HPLC. | |
23 kDa, observed by reducing SDS-PAGE. | |
CHO |
> 99.9% | |
25 KDa (reducing condition) |