Recombinant Rat Ciliary Neurotrophic Factor

SKU: orb1906109

Description

Recombinant Rat Ciliary Neurotrophic Factor

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg.
• Endotoxins: Less than 1 EU/ug of rRtCNTF as determined by LAL method.
• MW: Approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• Protein Sequence: AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4, 2 % trehalose.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CNTF

WB analysis of orb1906109.

Quick Database Links

UniProt

P20294