Recombinant Porcine Interleukin-1 Receptor Antagonist Protein

SKU: orb1906098

Description

Recombinant Porcine Interleukin-1 Receptor Antagonist Protein

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x104 IU/mg in the presence of 75 pg/ml rPoIL-1α.
• Molecular Weight: Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
• Protein Sequence: HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ
• Purity: > 97 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 0.1 EU/ug of rPoIL-1RA as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4, 1 mM DTT.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q29056