Recombinant Murine Platelet-derived Growth Factor-BB

SKU: orb1906165

Description

Recombinant Murine Platelet-derived Growth Factor-BB

Research Area

Stem Cell & Developmental Biology

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x106 IU/mg.
• Molecular Weight: Approximately 24.4 kDa, a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide (B chain).
• Protein Sequence: SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TIVTPRPVT
• Purity: > 98 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 0.1 EU/ug of rMuPDGF-BB as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P31240