Recombinant Murine Leukemia Inhibitory Factor

• Catalog Number: orb1906185
• Category: Proteins
• Description: Recombinant Murine Leukemia Inhibitory Factor
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.
• Purity: > 98 % by SDS-PAGE and HPLC analyses.
• MW: Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
• UniProt ID: P09056
• Protein Sequence: SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 x108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.
• Endotoxins: Less than 1 EU/ug of rMuLIF as determined by LAL method.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Alternative names: Differentiation-stimulating Factor, D factor, Mela
• Note: For research use only
• Expiration Date: 6 months from date of receipt.