Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12

SKU: orb1906153

Description

Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12

Research Area

Stem Cell & Developmental Biology; Musculoskeletal & Connective Tissue Research

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.
• Molecular Weight: Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.
• Protein Sequence: TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 0.1 EU/ug of rMuGDF-7/BMP-12 as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P43029