Recombinant Murine Fibroblast Growth Factor 21

SKU: orb1906171

Description

Recombinant Murine Fibroblast Growth Factor 21

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 x103 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.
• Molecular Weight: Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
• Protein Sequence: AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS
• Purity: > 97 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1 EU/ug of rMuFGF-21 as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 3 x PBS, pH 7.4.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q9JJN1