Recombinant Murine Ciliary Neurotrophic Factor

• Catalog Number: orb1906161
• Category: Proteins
• Description: Recombinant Murine Ciliary Neurotrophic Factor
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl, with 5 % Trehalose, 0.02 % Tween-20.
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• MW: Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 197 amino acids.
• UniProt ID: P51642
• Protein Sequence: AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES HYGAKQM
• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg.
• Endotoxins: Less than 1 EU/ug of rMuCNTF as determined by LAL method.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Note: For research use only
• Expiration Date: 6 months from date of receipt.