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Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | ELISA: Immobilized Recombinant Mouse TL1A(Cat#orb2994323) at 2μg/ml (100 μl/well) can bind Human TL1A Antibody (9H5)(Cat#orb2992933). The ED50 of Human TL1A Antibody (9H5)(Cat#orb2992933) is 1.73 ng/ml. (Regularly tested) . BLI: Loaded TL1A Antibody-Fc(Cat#orb2992933) on Protein A Biosensor, can bind Mouse TL1A-None(Cat#orb2994323) with an affinity constant of 12.8 pM as determined in BLI assay. (Regularly tested) |
| Tag | Tag free |
| Expression Region | Ile76-Leu252 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 20 KDa. Observed: 19 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC.(QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH 7.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor Necrosis Factor Ligand Superfamily Member 15; TNF Ligand-Related Molecule 1; Vascular Endothelial Cell Growth Inhibitor; TNFSF15; TL1; VEGI
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
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Mammalian
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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