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Recombinant Mouse ST2 Protein
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | ELISA: Immobilized Mouse IL-33(Cat#orb2994601) at 5μg/ml (100 μl/well) can bind Mouse ST2-Fc(Cat#orb2994391).The ED50 of Mouse ST2-Fc(Cat#orb2994391) is 0.25 ug/ml.. BLI: Loaded Human IL-33(Cat#orb2995234) on AR2G Biosensor, can bind Mouse ST2-Fc(Cat#orb2994391) with an affinity constant of 2.28nM as determined in BLI assay. Loaded Mouse IL-33(Cat#orb2994601) on AR2G Biosensor, can bind Mouse ST2-Fc(Cat#orb2994391) with an affinity constant of 1.49M as determined in BLI assay. |
| Tag | C-Fc |
| Molecular Weight | Predicted: 62.7 KDa. Observed: 90-110 KDa, reducing conditions |
| Expression Region | Ser27-Ala337 (Val192Ala) |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Recombinant Mouse ST2 Protein (orb2994391)
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