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Recombinant Mouse SEZ6 Protein

SKU: orb2992938

Description

Recombinant Mouse Seizure protein 6 is produced by our Mammalian expression system and the target gene encoding Leu20-His922 is expressed with a 9His tag at C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginMouse
Biological ActivityELISA: Immobilized Recombinant Mouse SEZ6 (C-9His)(Cat#orb2992938) at 5μg/ml (100 μl/well) can bind Anti-Human SEZ6 antibody (Human IgG1). The ED50 of Anti-Human SEZ6 antibody(Human IgG1) is 27.50 ng/ml. (Regularly tested). BLI: Loaded Anti-Human SEZ6 antibody (Human IgG1) on Pro-A Biosensor, can bind Recombinant Mouse SEZ6 (C-9His) (Cat#orb2992938) with an affinity constant of 0.312 uM as determined in BLI assay. (Regularly tested)
TagC-9His
Expression RegionLeu20-His922
Endotoxins< 1 EU/µg as determined by LAL test.
ConjugationUnconjugated
MWPredicted: 99.1 KDa. Observed: 140-220 KDa, reducing conditions
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Seizure protein 6; Sez6; SEZ6; Brain-specific receptor-like protein C

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  • Recombinant Human SEZ6 Protein [orb2992929]

    SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

    Predicted: 124.4 KDa. Observed: 135-214 KDa, reducing conditions

    1 mg, 10 μg, 50 μg, 500 μg
  • Mouse SEZ6 Protein, His Tag [orb1826800]

    Unconjugated

    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 98.7 kDa after removal of the signal peptide. The apparent molecular mass of mSEZ6-His is approximately 130-250 kDa due to glycosylation.

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Recombinant Mouse SEZ6 Protein (orb2992938)

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