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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | ELISA: Immobilized Recombinant Mouse SEZ6 (C-9His)(Cat#orb2992938) at 5μg/ml (100 μl/well) can bind Anti-Human SEZ6 antibody (Human IgG1). The ED50 of Anti-Human SEZ6 antibody(Human IgG1) is 27.50 ng/ml. (Regularly tested). BLI: Loaded Anti-Human SEZ6 antibody (Human IgG1) on Pro-A Biosensor, can bind Recombinant Mouse SEZ6 (C-9His) (Cat#orb2992938) with an affinity constant of 0.312 uM as determined in BLI assay. (Regularly tested) |
| Tag | C-9His |
| Expression Region | Leu20-His922 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 99.1 KDa. Observed: 140-220 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human SEZ6 Protein [orb2992929]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 124.4 KDa. Observed: 135-214 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μgMouse SEZ6 Protein, His Tag [orb1826800]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 98.7 kDa after removal of the signal peptide. The apparent molecular mass of mSEZ6-His is approximately 130-250 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg

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