Recombinant Mouse PD-L1 Protein

SKU: orb2994459

Description

Recombinant Mouse Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: ELISA: Immobilized Anti-Human PDL1 mAb-Fc(Cat#orb2993144) at 2μg/ml (100 μl/well)can bind Mouse PD-L1-His(Cat#orb2994459).The ED50 of Mouse PD-L1-His(Cat#orb2994459) is 23.35 ng/ml.. BLI: Loaded Mouse PD-L1-His(Cat#orb2994459) on HIS1K Biosensor, can bind Anti-Human PDL1 mAb-Fc(Cat#orb2993144) with an affinity constant of -3 nM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 25.6 KDa. Observed: 39-58 KDa, reducing conditions
• Expression Region: Phe19-Thr238
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Programmed cell death 1 ligand 1Cd274; programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;programmed death ligand 1;B7 homolog 1;B7-H1;CD274

Quick Database Links

UniProt

Q9EP73