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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | BLI: Loaded Biotinylated Human orb29948295-Fc-Avi(Cat#orb2993874) on Protein A Biosensor, can bind Mouse Mesothelin-His(Cat#orb2994270) with an affinity constant of 4.2 nM as determined in BLI assay. Loaded Anti-Human Mesothelin mAb-Fc(Cat#orb2992719) on Protein A Biosensor, can bind Human Mesothelin-His(Cat#orb2994270) with an affinity constant of 12.4 nM as determined in BLI assay. |
| Tag | C-6His |
| Expression Region | Asp298-Ser600 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 35 KDa. Observed: 38-55 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Mesothelin; Msln; Mes; Mpf
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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