Recombinant Mouse LEPR Protein

SKU: orb2993840

Description

Recombinant Mouse Leptin Receptor is produced by our Mammalian expression system and the target gene encoding Leu22-Gly839 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: ELISA: Immobilized Recombinant Mouse LEPR (C-10His)(Cat#orb2993840) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin(Cat#orb3002378)*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin(Cat#orb3002378) is 1.16 ng/ml.(Regularly tested).. BLI: Loaded Recombinant Mouse LEPR (C-10His)(Cat#orb2993840) on HIS1K Biosensor, can bind Recombinant Mouse Leptin(Cat#orb3002378) with an affinity constant of 0.196 nM as determined in BLI assay. (Regularly tested)
• Tag: C-10His
• Expression Region: Leu22-Gly839
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 93 KDa. Observed: 120-150 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295; LEPR; DB; OBR

Quick Database Links

UniProt

P48356