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Catalog Number | orb2816774 |
---|---|
Category | Proteins |
Description | Recombinant Mouse IL-4 |
Tag | C-terminal His Tag |
Reactivity | Mouse |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4. |
Purity | Greater than 90% as determined by SDS-PAGE. |
UniProt ID | P07750 |
MW | 21 kDa |
Tested applications | ELISA, WB |
Application notes | Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex |
Source | Mammalian Cells |
Expression Region | 21-140 |
Storage | The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. |
Alternative names | B cell stimulatory factor 1, Binetrakin, BSF 1, BS Read more... |
Note | For research use only |
> 97% as determined by SDS-PAGE and HPLC. | |
13.5 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
13.4 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
14.6 kDa | |
Mammalian cell |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 41.2 kDa after removal of the signal peptide. The apparent molecular mass of IL4-mFc is approximately 35-55 kDa due to glycosylation. | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |