Recombinant Mouse GPC3 Protein

SKU: orb2993897

Description

Recombinant Mouse Glypican-3 is produced by our Mammalian expression system and the target gene encoding Gln25-Met557 is expressed with a 6His tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Mouse
Tag	C-6His
Molecular Weight	Predicted: 61.6 KDa. Observed: 38&65-150 KDa, reducing conditions
Expression Region	Gln25-Met557
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; GPC3; OCI5

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Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 31.6 kDa after removal of the signal peptide. The apparent molecular mass of hFc-mGPC3(510-559) is approximately 25-55 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 10 μg, 50 μg, 100 μg

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Applications:
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MW:
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Sizes Available: 1 mg, 50 μg, 100 μg
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Applications:
ELISA, SDS-PAGE, WB
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>95% as determined by SDS-PAGE.
MW:
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Reactivity:
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Species/Host:
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Recombinant Mouse GPC3/Glypican 3 Protein, C-His [orb2831745]
Purity:
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Q8CFZ4

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