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Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | ELISA: Immobilized Recombinant Mouse GHR (C-Fc) (CM02) at 2μg/ml (100 μl/well) can bind Recombinant Mouse GH(orb2995242)*. *: Biotinylated by NHS-biotin prior to testing.The EC50 of Recombinant Mouse GH(orb2995242) is 3.54 ng/ml.(Regularly tested) |
| Tag | Tag free |
| Expression Region | Phe27-Phe216 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 22 KDa. Observed: 20 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC.(QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM HEPES, 4% Sucrose, 4% Mannitol, 0.02% Tween 80, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Protocol Information
Recombinant Mouse GH Protein (orb2995242)
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