Mouse FZD1

SKU: orb2994348

Description

Recombinant Mouse Frizzled (Drosophila) Homolog 1 is produced by our Mammalian expression system and the target gene encoding Val69-His248 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: ELISA: Immobilized Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) at 2 μg/ml (100 μl/well) can bind Recombinant Mouse FZD1 (C-Fc) (Cat#orb2994348)*. *: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Mouse FZD1 (C-Fc) (Cat#orb2994348) is 10.03 ng/ml.(Regularly tested).
• Tag: C-Fc
• Molecular Weight: Predicted: 46.8 KDa. Observed: 55-70 KDa, reducing conditions
• Expression Region: Val69-His248
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Frizzled-1; Fz-1; mFz1; Fzd1; Frizzled homolog 1

Quick Database Links

UniProt

O70421