Mouse Frizzled-2

SKU: orb2992555

Description

Recombinant Mouse Frizzled-2 is produced by our Mammalian expression system and the target gene encoding Gln29-Leu168 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: ELISA: Immobilized Human Frizzled receptors Antibody (Vantictumab, Research Grade) at 2μg/ml (100 μl/well) can bind Recombinant Mouse Frizzled-2 (C-Fc) (Cat#orb2992555). The ED50 of Recombinant Mouse Frizzled-2 (C-Fc) (Cat#orb2992555) is 0.65 μg/ml.(Regularly tested).. BLI: Loaded Human Frizzled receptors Antibody(Vantictumab,Research Grade)(Cat#orb2992588), can bind Recombinant Mouse Frizzled-2 (C-Fc) (C-Fc)(Cat#orb2992555) with an affinity constant of 0.215 μM as determined in BLI assay. (Regularly tested)
• Tag: C-Fc
• Molecular Weight: Predicted: 41 KDa. Observed: 45-60 KDa, reducing conditions
• Expression Region: Gln29-Leu168
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: ＜ 1 EU/μg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Frizzled-2; Fz-2; mFz2; Fzd2;frizzled (Drosophila) homolog 2

Quick Database Links

UniProt

Q9JIP6