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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | BLI: Loaded Recombinant Mouse CD32b(C-6His) (Cat#orb2994918) on HIS1K Biosensor, can bind Recombinant Mouse IgG1 Fc (Cat#orb2993959) with an affinity constant of 0.388 uM as determined in BLI assay. (Regularly tested) Loaded Mouse CD32-His(Cat#orb2994918) on HIS1K Biosensor, can bind Anti-Human HER2 mAb(Cat#GMP-A062) with an affinity constant of 1.14 uM as determined in BLI assay. |
| Tag | C-6His |
| Expression Region | Thr30-Pro210 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 21.6 KDa. Observed: 33 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII; IgG Fc receptor II beta; Lymphocyte antigen 17; Ly-17; CD32; Fcgr2; Fcgr2b; Ly-17
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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