Recombinant Mouse CD16 Protein

SKU: orb2994204

Description

Recombinant Mouse Low Affinity IgG Fc Receptor III is produced by our Mammalian expression system and the target gene encoding Ala31-Thr215 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: BLI: Loaded Anti-Human CD28 mAb(Cat#GMP-A063) on Protein A Biosensor, can bind Mouse CD16-His(Cat#orb2994204) with an affinity constant of 0.22 uM as determined in BLI assay. Loaded Recombinant Mouse IgG2a Fc (Cat#orb2993941) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.16 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Mouse IgG2b Fc (Cat#orb2993940) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.121 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Mouse IgG3 Fc (Cat#orb2993939) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.62 uM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 22 KDa. Observed: 35-40 KDa, reducing conditions
• Expression Region: Ala31-Thr215
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20 mM PB, 10% Sucrose, 8% Mannitol, 0.05% Tween 80, pH 7.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Low affinity immunoglobulin gamma Fc region receptor III; Fcgr3; Fc gamma Receptor III; CD-antigen 16; CD16; FcRIII; IgG Fc receptor III

Quick Database Links

UniProt

P08508