Recombinant Mouse B7-H4 Protein

SKU: orb2994220

Description

Recombinant Mouse B7 Homolog 4 is produced by our Mammalian expression system and the target gene encoding Phe29-Pro258 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: ELISA: Immobilized Anti-Human B7-H4 mAb(Cat#orb2993105) at 2μg/ml (100 μl/well) can bind Mouse B7-H4-Fc*(Cat#orb2994220).*: Biotinylated by NHS-biotin prior to testing The ED50 of Mouse B7-H4-Fc*(Cat#orb2994220) is 4.11 ng/ml.
• Tag: C-Fc
• Molecular Weight: Predicted: 52.2 KDa. Observed: 70-90 KDa, reducing conditions
• Expression Region: Phe29-Pro258
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

V-set domain containing T-cell activation inhibitor 1; B7 homolog 4; Protein B7S1; B7-H4; VTCN1

Quick Database Links

UniProt

AAH32925.1