Recombinant IL-1 beta (Interleukin-1 beta), Human, AF

• Catalog Number: orb1179080
• Category: Proteins
• Description: Recombinant IL-1 beta (Interleukin-1 beta), Human, AF
• Tag: His-tag at the N-terminus
• Reactivity: Human
• Form/Appearance: Lyophilized
• Buffer/Preservatives: The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
• Purity: > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
• Protein Sequence: MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus.
• Tested applications: ELISA
• Endotoxins: < 0.1 EU per 1 μg of the protein by the LAL method.
• Source: Escherichia coli
• Biological Activity: Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is < 10 pg/mL. The specific activity of recombinant human IL-1 beta is approximately > 1.5 x 108 IU/mg. Measure by its ability to induce IL-8 secretion in HT29 cells. The ED50 for this effect is 1.8-5.1 ng/mL.
• Storage: Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
• Alternative names: Catabolin, Lymphocyte-Activating Factor (LAF), End
• Note: For research use only
• Entrez: 3553