Recombinant Human/Mouse/Rat GDF-8 V2 Protein

SKU: orb2992531

Description

Recombinant Human/Mouse/Rat GDF-8 V2 is produced by our Mammalian expression system and the target gene encoding Asp267-Ser375 is expressed.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human/Mouse/Rat GDF-8 V2(Cat#orb2992531) at 2μg/ml (100 μl/well) can bind Anti-Human GDF-8 Antibody (TRE_bio, Research Grade). The ED50 of Anti-Human GDF-8 Antibody (TRE_bio, Research Grade) is 22.66ng/ml. (Regularly tested) Immobilized Recombinant Human ACVR2B (C-6His)(Cat#orb2995160) at 2μg/ml (100 μl/well) can bind Recombinant Human/Mouse/Rat GDF-8 V2(Cat#orb2992531)*. *: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human/Mouse/Rat GDF-8 V2(Cat#orb2992531) is 58.11ng/ml. (Regularly tested) Immobilized Recombinant Human ACVR2B (C-Fc-6His)(Cat#orb2993980) at 2μg/ml (100 μl/well) can bind Recombinant Human/Mouse/Rat GDF-8 V2(Cat#orb2992531)*. *: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human/Mouse/Rat GDF-8 V2(Cat#orb2992531) is 8.54ng/ml. (Regularly tested). Cell Based Assay: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 74 ng/ml. (Regularly tested)
• Tag: Tag free
• Molecular Weight: Predicted: 12.4kDa. Observed: 13-20kDa, reducing conditions
• Expression Region: Asp267-Ser375
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
• Endotoxins: ＜0.001EU/ug as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Growth/differentiation factor 8; MSTN; GDF-8; Myostatin; GDF8

Quick Database Links

UniProt

O14793