Recombinant Human VEGFR1 Protein

SKU: orb2994454

Description

Recombinant Human Vascular Endothelial Growth Factor Receptor 1 is produced by our Mammalian expression system and the target gene encoding Ser27-Asn756 is expressed with a human IgG1 Fc tag at the C-terminus.

Research Area

Cardiovascular Research

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human VEGF165 (Cat#orb2995236) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (Cat#orb2994454). The ED50 of Recombinant Human VEGFR1-Fc (Cat#orb2994454) is 36.4 ng/ml.. BLI: Loaded Biotinylated Human VEGF165 Protein(C-6His-Avi) (Cat#orb2992964) on HIS1K Biosensor, can bind Recombinant Human VEGFR1 (C-Fc) (Cat#orb2994454) with an affinity constant of 10.2 pM as determined in BLI assay. (Regularly tested)
• Tag: C-Fc
• Expression Region: Ser27-Asn756
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 109.3 KDa. Observed: 150-190 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5%Trehalose, 5%Mannitol, 0.01%Tween80, pH 7.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vascular endothelial growth factor receptor 1; VEGFR-1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; Vascular permeability factor receptor

Quick Database Links

UniProt

P17948