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Catalog Number | orb1905950 |
---|---|
Category | Proteins |
Description | Recombinant Human Trefoil Factor 2 |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
Purity | > 97 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 12.0 kDa, a single non-glycosylated polypeptide chain containing 106 amino acids, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds. |
UniProt ID | Q03403 |
Protein Sequence | EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x105 IU/mg. |
Endotoxins | Less than 1 EU/ug of rHuTFF2 as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Alternative names | Spasmolysin, Spasmolytic polypeptide, SP Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 13.2 kDa, a homodimer consisting of two 60 amino acid polypeptide chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds. | |
Escherichia coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
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