Recombinant Human Trefoil Factor 3

SKU: orb1905949

Description

Recombinant Human Trefoil Factor 3

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg.
• Molecular Weight: Approximately 13.2 kDa, a homodimeric protein consisting of two 59 amino acid chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
• Protein Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
• Purity: > 97 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1 EU/ugof rHuTFF3 as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Intestinal Trefoil Factor, hITF, Polypeptide P1.B, hP1.B

Quick Database Links

UniProt

Q07654