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Recombinant Human Transforming Growth Factor - beta 2
Description
Images & Validation
−Key Properties
−| Source | Mouse myeloma cell line, NS0 |
|---|---|
| Expression System | Mouse myeloma cell line, NS0 |
| Biological Activity | Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.025-0.25 ng/ml. |
| Molecular Weight | Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 12 kDa under reducing conditions, a disulfide-linked homodimer of two 112 amino acid glycosylated polypeptide chains. |
| Protein Sequence | Ala303-Ser414; Accession # P61812 |
| Purity | > 97 % by SDS-PAGE analyses. |
| Endotoxins | Less than 0.1 EU/ug of rHuTGF-β2 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from 0.2 μm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
Recombinant Human Transforming Growth Factor - beta 2 (orb1906260)
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