Recombinant Human TIM-3 Protein

SKU: orb2995123

Description

Recombinant Human T-Cell Membrane Protein 3 is produced by our Mammalian expression system and the target gene encoding Ser22-Arg200 is expressed with a 6His tag at the C-terminus.

Research Area

Immunology & Inflammation

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-Human TIM-3 mAb(Cat#orb2993103) at 2μg/ml (100 μl/well) can bind Human TIM-3-His (Cat#orb2995123). The ED50 of Recombinant Human TIM-3-His (Cat#orb2995123) is 8.51ng/ml.
• Tag: C-6His
• Molecular Weight: Predicted: 20.86 KDa. Observed: 37 KDa, reducing conditions
• Expression Region: Ser22-Arg200
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hepatitis A Virus Cellular Receptor 2; HAVcr-2; T-Cell Immunoglobulin and Mucin Domain-Containing Protein 3; TIMD-3; T-Cell Membrane Protein 3; TIM-3; HAVCR2; TIM3; TIMD3

Quick Database Links

UniProt

AAL65157.1