Recombinant Human TIGIT Protein

SKU: orb2994151

Description

Recombinant Human T-cell Immunoreceptor With Ig And ITIM Domains is produced by our Mammalian expression system and the target gene encoding Met22-Pro141 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Recombinant Mouse PVR (C-6His) (Cat#orb2992802) on HIS1K Biosensor, can bind Recombinant Human TIGIT (C-Fc) (Cat#orb2994151) with an affinity constant of 17.6 nM as determined in BLI assay. (Regularly tested) Loaded Human PVR-His(Cat#orb2993514) on HIS1K Biosensor, can bind Human TIGIT-Fc(Cat#orb2994151) with an affinity constant of 8.58 nM as determined in BLI assay.
• Tag: C-Fc
• Expression Region: Met22-Pro141
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 39.7 KDa. Observed: 40-55 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3; Tigit; VSIG9; VSTM3

Quick Database Links

UniProt

Q495A1