Recombinant Human TFIIB Protein

SKU: orb2995165

Description

Recombinant Human Transcription Initiation Factor IIB is produced by our E.coli expression system and the target gene encoding Met1-Leu316 is expressed with a GST tag at the N-terminus.

Key Properties

• Expression System: E.coli
• Biological Origin: Human
• Tag: N-GST
• Molecular Weight: Predicted: 61.64 KDa. Observed: 62 KDa, reducing conditions
• Expression Region: Met1-Leu316
• Purity: SDS-PAGE: Greater than 80% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcription Initiation Factor IIB; General Transcription Factor TFIIB; S300-II; GTF2B; TF2B; TFIIB

Quick Database Links

UniProt

Q00403