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Catalog Number | orb1905952 |
---|---|
Category | Proteins |
Description | Recombinant Human Syndecan-4 |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Purity | > 95 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 13.9 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. But it migrates with an apparent molecular mass of 22 kDa in SDS-PAGE. |
UniProt ID | P31431 |
Protein Sequence | ESIRETEVID PQDLLEGRYF SGALPDDEDV VGPGQESDDF ELSGSGDLDD LEDSMIGPEV VHPLVPLDNH IPERAGSGSQ VPTEPKKLEE NEVIPKRISP VEESEDVSNK VSMSSTVQGS NIFERTE |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The specific activity is determined by binding ability in a functional ELISA. Immobilized rHuSYND4 at 500 ng/ml (100 μl/well) can bind rHubFGF with a linear range of 0.1-10 ng/ml. |
Endotoxins | Less than 0.1 EU/ugof rHuSYND4 as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
HPLC, SDS-PAGE | |
Unconjugated | |
> 90% by SDS-PAGE and HPLC analyses | |
15.4 kDa |
98.00% | |
Approxiamtely 15.3 kDa |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
15.7 kDa | |
E.Coli |
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