Recombinant Human soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A

SKU: orb1906292

Description

Recombinant Human soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A

Research Area

Cancer Biology; Immunology & Inflammation; Cell Biology

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.05 μg/ml, corresponding to a specific activity of > 2 x104IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha.
• Molecular Weight: Approximately 21.2 kDa, a single non-glycosylated polypeptide chain containing 190 amino acids.
• Protein Sequence: IYPSGVIGLV PHLGDREKRD SVCPQGKYIH PQNNSICCTK CHKGTYLYND CPGPGQDTDC RECESGSFTA SENHLRHCLS CSKCRKEMGQ VEISSCTVDR DTVCGCRKNQ YRHYWSENLF QCFNCSLCLN GTVHLSCQEK QNTVCTCHAG FFLRENECVS CSNCKKSLEC TKLCLPQIEN VKGTEDSGTT
• Purity: > 97 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 0.1 EU/ug of rHusTNF RI/TNFRSF1A as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P19438