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Human SIRPA
SKU: orb2995109
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Anti-Human SIRPA mAb-Fc(Cat#orb2993142) on Protein A Biosensor, can bind Human SIRPA-His(Cat#orb2995109) with an affinity constant of 10.1 nM as determined in BLI assay. |
| Tag | C-6His |
| Molecular Weight | Predicted: 38.31 KDa. Observed: 45-65 KDa, reducing conditions |
| Expression Region | Glu31-Arg370 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; SHP Substrate 1; SHPS-1; Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs; Bit; CD172 Antigen-Like Family Member A; Inhibitory Feceptor SHPS-1; Macrophage Fusion Receptor; MyD-1 Antigen; Signal-Regulatory Protein Alpha-1; Sirp-Alpha-1; Signal-Regulatory Protein Alpha-2; Sirp-Alpha-2; Signal-Regulatory Protein Alpha-3; Sirp-Alpha-3; p84; CD172a; SIRPA; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP
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Human SIRPa Protein, hFc-His Tag [orb689370]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 70-98 kDa after removal of the signal peptide.
Mammalian
10 μg, 50 μg, 100 μg Mouse SIRPA [orb2994264]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 64.8 KDa. Observed: 115-125 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman SIRPA [orb2994265]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 64.1 KDa. Observed: 85-105 KDa, reducing conditions
500 μg, 1 mg, 10 μg, 50 μg
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Human SIRPA (orb2995109)
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