Recombinant Human SEMA3A Protein

SKU: orb2994243

Description

Recombinant Human Semaphorin 3A is produced by our Mammalian expression system and the target gene encoding Lys26-Phe546 is expressed with a 6His-Flag tag at the N-terminus.

Research Area

Neuroscience

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Tag: N-6His-Flag
• Expression Region: Lys26-Phe546
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 61.3 KDa. Observed: 70-75 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 8.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Semaphorin III; SEMA3A; SEMAD; Hsema-I; SEMA1; SemD; Semad; coll-1; Hsema-III; Sema III

Quick Database Links

UniProt

Q14563