Recombinant Human S100B protein

Catalog Number: orb1906224

Catalog Number	orb1906224
Category	Proteins
Description	Recombinant Human S100B protein
Form/Appearance	Lyophilized from a 0.2 mm filtered concentrated solution in PBS, pH 7.4.
Purity	> 95 % by SDS-PAGE.
MW	Approximately 10.6 kDa, a single non-glycosylated polypeptide chain containing 91 amino acids
Protein Sequence	SELEKAMVAL IDVFHQYSGR EGDKHKLKKS ELKELINNEL SHFLEEIKEQ EVVDKVMETL DNDGDGECDF QEFMAFVAMV TTACHEFFEH E
Source	Escherichia coli
Expression System	Escherichia coli
Biological Activity	Measured in a cell proliferation assay using human peripheral blood monocytes. The ED50 for this effect is 1-4 μg/mL.
Endotoxins	Less than 1.0 EU/ug of rHuS100B as determined by LAL method.
Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Note	For research use only
Expiration Date	6 months from date of receipt.

Purity:	> 95% by SDS-PAGE.
Source:	KMP1784, Recombinant Human S100 Calcium Binding Protein B/S100B Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Glu92) of human S100 Calcium Binding Protein B/S100B (Accession #P04271) fused with an initial Met at the N-terminus and a 6×His Tag at the N-terminus.

Sizes Available: 500 μg, 50 μg

Applications:	ELISA, MS, SDS-PAGE, WB
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Source:	E. coli

Purity:	≥90% as determined by SDS-PAGE
Source:	This protein contains the human S100B(Met1-Glu92) was fused with the N-terminal His Tag and expressed in E. coli.