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Description
Research Area
Signal Transduction
Images & Validation
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Measured in a cell proliferation assay using human peripheral blood monocytes. The ED50 for this effect is 1-4 μg/mL. |
| Endotoxins | Less than 1.0 EU/ug of rHuS100B as determined by LAL method. |
| MW | Approximately 10.6 kDa, a single non-glycosylated polypeptide chain containing 91 amino acids |
| Purity | > 95 % by SDS-PAGE. |
| Protein Sequence | SELEKAMVAL IDVFHQYSGR EGDKHKLKKS ELKELINNEL SHFLEEIKEQ EVVDKVMETL DNDGDGECDF QEFMAFVAMV TTACHEFFEH E |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 mm filtered concentrated solution in PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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