Recombinant Human RGMB Protein

SKU: orb2992923

Description

Recombinant Human Repulsive guidance molecule B is produced by our Mammalian expression system and the target gene encoding Gly46-Ser411 is expressed with a human IgG1 Fc tag at C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human RGMB (C-Fc) (Cat#orb2992923) at 5 μg/ml (100 μl/well) can bind Recombinant Human BMP-4 (Cat#orb3002515)*. *: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human BMP-4 (Cat#orb3002515) is 0.18 μg/ml. (Regularly tested).
• Tag: C-Fc
• Molecular Weight: Predicted: 67.2 KDa. Observed: (16-21)&(40-46)&(57-71) KDa, reducing conditions
• Expression Region: Gly46-Ser411
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Repulsive guidance molecule B; RGMB; DRG11-responsive axonal guidance and outgrowth of neurite; DRAGON.

Quick Database Links

UniProt

Q6NW40