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Human RANK L
SKU: orb2994417
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Human RANK-His(Cat#orb2994416) on HIS1K Biosensor, can bind Human RANK L (Cat#orb2994417) with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human OPG-Fc (Cat#orb3002306) on Pro A Biosensor, can bind Human RANKL (Cat#orb2994417) with an affinity constant of 1.83 pM as determined in BLI assay. |
| Tag | Tag free |
| Molecular Weight | Predicted: 22.4 KDa. Observed: 20 KDa, reducing conditions |
| Expression Region | Ile140-Asp317 |
| Purity | SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−CD254; ODF; OPGL; RANK L; TNFSF11; CD254; Osteoclast differentiation factor; Receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor ligand superfamily member 11
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Human RANK L (orb2994417)
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