Recombinant Human R-spondin-1 (RSPO1) (Active)

• Catalog Number: orb2657889
• Category: Proteins
• Description: Recombinant Human R-spondin-1 (RSPO1) (Active)
• Tag: Tag free
• Form/Appearance: Lyophilized powder
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4
• Purity: Greater than 95% as determined by SDS-PAGE.
• Protein Sequence: SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA
• Protein Length: Full Length of Mature Protein
• UniProt ID: Q2MKA7
• MW: 26.7 kDa
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Endotoxins: ≤10 EU/mg by the LAL method
• Source: Mammalian cell
• Biological Origin: Homo sapiens (Human)
• Biological Activity: Measured by its ability to induce Topflash reporter activity in CHO cells in the presence of 50ng/ml wnt3a. The expected ED50 is＜1.0 μg/ml.
• Expression Region: 21-263aa
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Alternative names: RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ409
• Note: For research use only
• Expiration Date: 6 months from date of receipt.